The Method and Study of Detecting Phenanthrene in Seawater Based on a Carbon Nanotube–Chitosan Oligosaccharide Modified Electrode Immunosensor

Phenanthrene (PHE), as a structurally simple, tricyclic, polycyclic aromatic hydrocarbon (PAHs), is widely present in marine environments and organisms, with serious ecological health impacts. It crucial to study fast simple high-sensitivity detection methods for phenanthrene seawater the environment human body. In this paper, immunosensor was prepared by using multi-wall carbon nanotube (MWCNTs)-chitosan oligosaccharide (COS) nanocomposite membrane loaded antibody. The principle based on antibody–antigen reaction immune reaction, strong electron transfer ability of multi-walled nanotubes, coupled chitosan oligosaccharides an excellent film formation biocompatibility, amplify signal. content studied via differential pulse voltammetry (DPV) potassium ferricyanide system redox probe. antibody concentration, pH value, probe concentration were optimized. Under optimal experimental conditions, response peak current inversely proportional phenanthrene, range from 0.5 ng·mL−1 80 ng·mL−1, limit 0.30 ng·mL−1. sensor successfully applied water, recovery rate 96.1~101.5%, provided stable, sensitive, accurate method real-time monitoring environments.